Method development for speciation analysis of mercury and selenium in sediments and biota

Method development for speciation analysis of mercury and selenium in sediments and biota

Project Summary

This research project comprises two parts, describing the development of methods for the extraction and measurement of mercury and selenium species in marine sediments and biota. Mercury species in sediments and fish tissues were extracted using 2-mercaptoethanol and analysed by reverse phase chromatography-inductively coupled plasma mass spectrometry (ICPMS). Selenoamino acids in marine animal tissues were analysed after proteolytic enzymatic digestion and analysis using anion and cation exchange chromatography, size exclusion chromatography and reverse phase chromatography-ICPMS.